About input:
- SEQUTI only accept nuclear acid sequence, the characters it accepts are
regualr base symbol : A G C T U
degenerate base symbol : R Y M K S W B D H V N
special symbol : ^ ( ) { }
other characters or numbers in your sequence will be ignored.
- SEQUTI can only process one sequence at a time. Don't put multiple sequences at the same time.
- SEQUTI does not support FASTA format, if you try to input FASTA format, it will give you WRONG result! But if you take out the first row of your FASTA format (the row contains
" > ") and your FASTA format contains only one sequence, Sequti will work fine.
Complement rule:
- A->T, G->C, C->G, T->A, U->A
- R->Y, Y->R, M->K, K->M, S->S, W->W, B->V, V->B, D->H, H->D, N->N
About output:
- Each row has 60 bases. Even you have special symbol ( Ex.parentheses) in that row, the row will still contain exactly 60 bases.
- Regular base symbol will be lower case. Degenerate base symbol will be upper case.
- SEQUTI will automatically reverse the direction of your parentheses after it performs Reverse or Reverse complement function.
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